Boxall J D, Proops D W, Michaels L
Department of Histopathology, University College and Middlesex School of Medicine, London, England.
J Otolaryngol. 1990 Feb;19(1):57-61.
The en masse locomotion in tissue culture of stratified squamous epithelium of the tympanic membrane and cholesteatoma is a unique feature of these epithelia. A scanning electron microscopy study of cultures was carried out to seek features of surface architecture that might throw light on this special activity. Differences from cultures of non-migratory stratified squamous epithelium include the following: numerous lamellae emanating from the top surface of cells at the leading edge, a ridge of spent leading edge cells behind that edge, a cap of keratin behind the latter and large balloon-like swelling of cells is prevalent in the trailing edge. It is suggested that differentiation of migrating stratified squamous epithelium in tissue culture is towards specialized cells active in migration as shown by the leading and trailing edge cells, as well as towards keratinization as shown by the keratin cap.
鼓膜和胆脂瘤的复层鳞状上皮在组织培养中的集体运动是这些上皮的一个独特特征。对培养物进行了扫描电子显微镜研究，以寻找可能有助于解释这种特殊活动的表面结构特征。与非迁移性复层鳞状上皮培养物的差异包括：前缘细胞顶面发出大量薄片，该边缘后方有一排用过的前缘细胞形成的嵴，后者后方有角质形成帽，并且后缘细胞普遍存在大的气球样肿胀。有人提出，组织培养中迁移性复层鳞状上皮的分化既朝着如前缘和后缘细胞所示的在迁移中活跃的特化细胞方向，也朝着如角质帽所示的角质化方向。
