胚胎干细胞试验。
The embryonic stem cell test.
作者信息
Schulpen Sjors H W, Piersma Aldert H
机构信息
Laboratory for Health Protection Research-National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands.
出版信息
Methods Mol Biol. 2013;947:375-82. doi: 10.1007/978-1-62703-131-8_27.
The embryonic stem cell test is an animal-free alternative test method for developmental toxicity. Mouse embryonic stem cells are cultured in a hanging drop method to form embryoid bodies. These embryoid bodies, when plated on tissue culture dishes, differentiate to form contracting myocardial cell foci within 10 days. Inhibition of cardiomyocyte differentiation by test compounds serves as the end point of the assay, as monitored by counting contracting muscle foci under the microscope.
胚胎干细胞试验是一种用于发育毒性检测的无动物替代试验方法。小鼠胚胎干细胞采用悬滴法培养以形成胚状体。这些胚状体接种到组织培养皿上后，在10天内会分化形成收缩性心肌细胞灶。受试化合物对心肌细胞分化的抑制作用作为该检测的终点，通过在显微镜下计数收缩性肌肉灶来监测。
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