Langlois M, Gibert R, Aymard M, Denis J
Ann Microbiol (Paris). 1979 Aug-Sep;130B(2):245-61.
Two methods for the differentiation of herpes simplex virus (HSV)--rct and neutralization by specific antisera--have been applied to 8 prototype strains and 105 strains isolated from various clinical cases. The evaluation of the II/I index allows to choose the most specific rabbit antisera. The rct 40 degrees 3 C marker test gives comparable results for HSV grown in RK13 and human fibroblasts as well; it is in agreement with the immunological test in 48% of the cases. The delta tc values used to express the immunological typing allow to differentiate 76% of the strains (types 1 and 2). The others (24%) are equally neutralized by both specific antisera; they represent "intermediate" strains. The infection of man and animal by HSV (type 1, 2 or "intermediate") also produces heterospecific antibodies in variable amount. From clinical and immunological data rises the hypothesis that "intermediate" strains derive from HSV1 strains by antigenic variation during a persistent infection.
两种鉴别单纯疱疹病毒（HSV）的方法——空斑减少中和试验（rct）和用特异性抗血清进行中和试验，已应用于8株原型毒株和从各种临床病例中分离出的105株毒株。对II/I指数的评估有助于选择最特异的兔抗血清。40℃ 3小时的rct标记试验对在RK13细胞和人成纤维细胞中生长的HSV也能得出可比的结果；在48%的病例中，该试验结果与免疫试验结果一致。用于表达免疫分型的δtc值能够鉴别76%的毒株（1型和2型）。其余24%的毒株能被两种特异性抗血清同等程度地中和；它们代表“中间型”毒株。人及动物感染HSV（1型、2型或“中间型”）也会产生数量不等的异种特异性抗体。从临床和免疫学数据中得出这样的假说：“中间型”毒株是在持续性感染过程中，由HSV-1毒株通过抗原变异产生的。
