Bystrická M, Petríková M, Zatovicová M, Soláriková L, Kostolanský F, Mucha V, Russ G
Institute of Virology, Bratislava, Slovak Republic.
Acta Virol. 1997 Feb;41(1):5-12.
The relative importance of the humoral immune response to various antigenic sites on the glycoproteins C and B (gC, gB) of herpes simplex virus (HSV) was evaluated in BALB/c and DBA/2 mice passively immunized with monoclonal antibodies (MoAbs) and then challenged with lethal dose of infectious virus. Eight MoAbs to three topographically distinct antigenic sites on gC and eight MoAbs to two distinct antigenic sites on gB were selected. The results indicated that any antigenic site on gC and gB contains epitopes for the protective immunity. However, individual MoAbs to different epitopes of the same antigenic site (i.e. antigenic site III on gC, and antigenic site II on gB) varied extremely in their protective ability. The protection did not correlate with the virus neutralization in vitro whereas it correlated significantly with the immune cytolysis in the presence of complement. The information about protective epitopes is essential for understanding the immunology of HSV infection at molecular level and may have implications for the design of HSV vaccine.
通过用单克隆抗体（MoAbs）被动免疫BALB/c和DBA/2小鼠，然后用致死剂量的感染性病毒进行攻击，评估了体液免疫反应对单纯疱疹病毒（HSV）糖蛋白C和B（gC、gB）上各种抗原位点的相对重要性。选择了针对gC上三个拓扑不同抗原位点的八种MoAbs和针对gB上两个不同抗原位点的八种MoAbs。结果表明，gC和gB上的任何抗原位点都包含保护性免疫的表位。然而，针对同一抗原位点不同表位的单个MoAbs（即gC上的抗原位点III和gB上的抗原位点II）的保护能力差异极大。保护作用与体外病毒中和无关，而与补体存在下的免疫细胞溶解显著相关。关于保护性表位的信息对于在分子水平上理解HSV感染的免疫学至关重要，并且可能对HSV疫苗的设计有影响。
