Khan Majida, Zafar Shaheen, Syed Serajuddaula
Sindh Institute of Reproductive Medicine (SIRM) Karachi, Pakistan.
J Pak Med Assoc. 2012 Sep;62(9):887-92.
To assess the survival of freezing cleaved human embryos through vitrification.
The prospective study was conducted at the Karachi-based Sindh Institute of Reproductive Medicine between June 2008 and June 2009. The cryopreservation of embryos being a new technology in Pakistan, only 19 couples, picked through convenience sampling, comprised the study population. The couples were treated for infertility by in virto fertilisation (IVF) or intracytoplasmic sperm injection (ICSI); 125 surplus embryos were vitrified. Subsequently, 15 embryos were thawed, and transferred in a controlled cycle. SPSS version 11 was used for statistical analysis.
After the surplus embryos were vitrified and subsequently thawed and transferred, the survival of the embryos was assessed by the number of blastomeres that were intact. The overall embryo survival rate was (14/15) 93.33%.
Vitrification is a simple procedure that requires less time and is likely to become safer and more cost-effective with time. Survival rate after thawing and preserving is high, but comparative success rates in terms of pregnancy and taking-home-baby rates are yet to be established in Pakistan.
评估玻璃化冷冻切割人类胚胎的存活率。
前瞻性研究于2008年6月至2009年6月在卡拉奇的信德生殖医学研究所进行。胚胎冷冻保存技术在巴基斯坦是一项新技术，通过便利抽样选取了19对夫妇作为研究对象。这些夫妇通过体外受精（IVF）或卵胞浆内单精子注射（ICSI）治疗不孕症；125个多余胚胎被玻璃化冷冻。随后，解冻15个胚胎，并在一个控制性周期中进行移植。使用SPSS 11版进行统计分析。
多余胚胎经玻璃化冷冻、随后解冻并移植后，通过完整卵裂球的数量评估胚胎的存活率。总体胚胎存活率为（14/15）93.33%。
玻璃化冷冻是一个简单的过程，所需时间较少，并且随着时间推移可能会变得更安全、更具成本效益。解冻和保存后的存活率很高，但在巴基斯坦，关于妊娠率和抱婴回家率的比较成功率尚未确定。
