Department of Analytical Chemistry, Research Institute of Fine Chemistry and Nanochemistry, Campus of Rabanales, Marie Curie Building (Annex), University of Cordoba, 14071-Cordoba, Spain.
J Agric Food Chem. 2012 Nov 28;60(47):11741-7. doi: 10.1021/jf303919q. Epub 2012 Nov 19.
The usefulness of Tb(4)O(7) nanoparticles (NPs) as analytical reagents using sensitized luminescence as a detection system is described for the first time, and the results obtained are compared with those obtained using Tb(III) ions. Two drugs used in veterinary practice, namely, lasalocid (LAS) and salicylate (SAL), have been chosen as model analytes to carry out this study. The experimental conditions for these systems have been optimized, and their analytical features were obtained. The detection limits obtained for LAS and SAL using Tb(4)O(7) NPs were 1.0 and 4.0 ng mL(-1), respectively, which were comparable to those obtained using Tb(III) ions: 1.8 and 1.0 ng mL(-1), respectively. However, precision data, with relative standard deviation values in the range 2.3-3.8% using the NPs and 3.5-6.5% using Tb(III) ions, were slightly better for LAS with Tb(4)O(7) NPs. The practical analytical usefulness of Tb(4)O(7) NPs as luminescent reagents has been shown by performing the determination of LAS in tap water, feed premix, and egg samples, obtaining recoveries in the range of 80.0-105.0%.
首次描述了 Tb(4)O(7)纳米粒子 (NPs) 作为分析试剂的用途，使用敏化发光作为检测系统，并将得到的结果与使用 Tb(III)离子得到的结果进行了比较。选择兽医实践中使用的两种药物，即莱萨洛西（LAS）和水杨酸盐（SAL），作为模型分析物进行这项研究。优化了这些体系的实验条件，并获得了它们的分析特征。使用 Tb(4)O(7) NPs 检测 LAS 和 SAL 的检测限分别为 1.0 和 4.0 ng mL(-1)，与使用 Tb(III)离子获得的值相当：分别为 1.8 和 1.0 ng mL(-1)。然而，使用 NPs 的相对标准偏差值在 2.3-3.8%范围内，使用 Tb(III)离子的相对标准偏差值在 3.5-6.5%范围内，对于 LAS 来说，使用 Tb(4)O(7) NPs 的精密度数据略好一些。通过在自来水中、饲料预混料和鸡蛋样品中进行 LAS 的测定，显示了 Tb(4)O(7) NPs 作为发光试剂的实际分析用途，回收率在 80.0-105.0%范围内。
