Department of Microbiology, School of Life Sciences, North Maharashtra University, P.O. Box-80, Jalgaon 425 001, India.
Food Chem. 2011 Jul 15;127(2):387-93. doi: 10.1016/j.foodchem.2010.12.126. Epub 2011 Jan 8.
The aim of this study was to characterise siderophores of a culture bearing better probiotic qualities of human origin. In this direction, bacterial strain ST2 was isolated from faecal sample of 6-month-old healthy infant, which showed close similarity with Escherichia coli based on 16S r-RNA gene sequence analysis and biochemical characteristics. It was tested for siderophore production under in vitro iron limiting intestinal conditions. The siderophore produced was catecholate type of 2,3-dihydroxybenzoylserine on the basis of FTIR, NMR and mass spectra analysis. The strain obeyed probiotic properties as per WHO and FAO guidelines. It showed excellent tolerance in partially simulated gastro-intestinal tract conditions, non-haemolytic nature, susceptibility to many antibiotics tested and antimicrobial activity against enteric pathogens like Salmonella typhimurium, Streptococcus pyogenes, and Staphylococcus aureus. Hence, this culture could be a possible probiotic candidate in iron nutrition, but only after conducting proper animal and human trials.
本研究旨在对具有更好人类来源益生菌特性的培养物中的铁载体进行特征分析。为此，从 6 个月大健康婴儿的粪便样本中分离出了 ST2 菌株，根据 16S r-RNA 基因序列分析和生化特性，该菌株与大肠杆菌具有密切相似性。在体外铁限制肠道条件下对其铁载体的产生进行了测试。根据傅里叶变换红外光谱（FTIR）、核磁共振（NMR）和质谱分析，所产生的铁载体为 2,3-二羟基苯甲酰丝氨酸型。该菌株符合世界卫生组织（WHO）和粮农组织（FAO）的益生菌特性标准。它在部分模拟胃肠道条件下表现出出色的耐受性、非溶血性、对许多测试抗生素的敏感性以及对肠道病原体（如鼠伤寒沙门氏菌、化脓性链球菌和金黄色葡萄球菌）的抗菌活性。因此，这种培养物可能是铁营养方面的一种潜在益生菌候选物，但仅在进行适当的动物和人体试验后才能使用。
