Panda Smita H, Goli Jyothsna Khanna, Das Sushrirekha, Mohanty Nakulananda
Department of Zoology, North Orissa University, Baripada, Odisha-757003, India.
Department of Microbiology, Osmania University, Hyderabad, Andhra Pradesh-500007, India.
AIMS Microbiol. 2017 Feb 22;3(1):88-107. doi: 10.3934/microbiol.2017.1.88. eCollection 2017.
The aim of the study was to characterize the probiotic qualities and siderophore production of and isolates for possible application for iron nutrition in human and animals strains were selectively isolated from different dairy sources and infant faecal matter. Isolates SB10, JC13 and IFM22 were found to produce maximum siderophore ranging from 65-90% at an optimum pH 7, incubation period of 96 h, agitation speed of 150 rpm and inoculum volume of 15%. SB10 and JC13 were found to show high homology with spp. and IFM22 with spp., using partial 16S rRNA sequencing and biochemical characterization. All the three isolates produced hydroxymate type of siderophores under iron stressed conditions and screened for probiotic characters as per WHO guidelines. Strains have shown excellent tolerance to acid, bile salt, sodium chloride and phenol. They were non-haemolytic in nature and exhibited high hydrophobicity and autoaggregation. Our isolates proved to be potent probiotic strains due to their survival under highly acidic conditions and higher tolerance to bile salt. In addition, its colonization efficiency was proved by exhibiting high autoaggregation and hydrophobicity.
本研究的目的是对从不同乳制品来源和婴儿粪便中选择性分离出的菌株的益生菌特性和铁载体产生情况进行表征，以便可能应用于人和动物的铁营养。分离株SB10、JC13和IFM22在最适pH值7、培养96小时、搅拌速度150转/分钟和接种量15%的条件下，铁载体产量最高，范围为65%-90%。通过部分16S rRNA测序和生化表征发现，SB10和JC13与 属的菌株具有高度同源性，IFM22与 属的菌株具有高度同源性。在铁胁迫条件下，所有这三株分离株均产生羟基肟酸盐型铁载体，并按照世界卫生组织的指南对益生菌特性进行了筛选。这些菌株对酸、胆盐、氯化钠和苯酚表现出优异的耐受性。它们本质上是非溶血的，表现出高疏水性和自聚集性。由于我们的分离株在高酸性条件下存活且对胆盐具有更高的耐受性，因此被证明是有效的益生菌菌株。此外，通过表现出高自聚集性和疏水性证明了其定殖效率。
