Lehtinen P, Kulonen E
Biochim Biophys Acta. 1979 Aug 29;564(1):132-40. doi: 10.1016/0005-2787(79)90194-1.
The supernatant from SiO2-treated macrophages increased the incorporated radioactivity of collagen in the incubated experimental granulation tissue slices, especially in the rough endoplasmic reticulum fraction, markedly over that in the respective control preparation. The effect was observed after a 20 min incubation and increases linearly at least up to 3 h. The amount of RNA and phospholipids in the rough endoplasmic reticulum, calculated per protein, increased simultaneously in the incubated slices. The incorporation of the radioactive precursors into DNA in the slices and in the nuclei from proliferating granulation tissue was enhanced significantly by the soluble fraction from SiO2-treated macrophages. This effect could be seen after a 40 min incubation in the slices and after 5 min in the nuclei, when the incorporated radioactivity in DNA began to decrease in the control experiments.
经二氧化硅处理的巨噬细胞的上清液增加了孵育的实验性肉芽组织切片中胶原蛋白的掺入放射性，尤其是在粗面内质网部分，明显高于各自对照制剂中的水平。在孵育20分钟后观察到这种效应，并且至少在3小时内呈线性增加。以蛋白质计算，孵育切片中粗面内质网中的RNA和磷脂量同时增加。经二氧化硅处理的巨噬细胞的可溶部分显著增强了放射性前体掺入切片和增殖性肉芽组织细胞核中的DNA的过程。在切片中孵育40分钟后以及在细胞核中孵育5分钟后可以看到这种效应，此时对照实验中DNA的掺入放射性开始下降。
