Aho S, Lehtinen P, Kulonen E
Acta Pathol Microbiol Immunol Scand C. 1982 Jun;90(3):147-54. doi: 10.1111/j.1699-0463.1982.tb01431.x.
RNAase isolated from macrophage culture medium was labelled with 3H-acetic anhydride. The acetylation density of 1.8 acetyl residues/10(4) dalton RNAase was achieved. After just 5 mins' incubation of granulation-tissue slices with 3H-Ac-RNAase, the enzyme had entered fibroblasts, and after 20 min there was only slight increase of the radioactivity. After 1 h incubation 32% of the ingested RNAse was in the nuclei, 23% in the cytosol and about 2% in RER. After the same period of incubation with 3H-Ac-RNAase, 47% of nuclear RNA of the fibroblasts was lost, as compared with the control incubation, and the amount of cytosol RNA increased by about 40%. During a 3-h incubation, in low concentrations, the biologically active macrophage RNAase stimulated. 3H-thymidine incorporation into DNA of proliferative granulation-tissue slices. Thus it was concluded that the fibrogenic effect of the macrophage RNAase on the granulation-tissue fibroblasts takes place through the RNA metabolism and the main target of the enzyme in the fibroblasts is the nuclear RNA.
从巨噬细胞培养基中分离出的核糖核酸酶用³H - 乙酸酐进行标记。实现了每10⁴道尔顿核糖核酸酶有1.8个乙酰基残基的乙酰化密度。用³H - 乙酰化核糖核酸酶孵育肉芽组织切片仅5分钟后，该酶就已进入成纤维细胞，20分钟后放射性仅有轻微增加。孵育1小时后，摄入的核糖核酸酶中有32%在细胞核中，23%在细胞质溶胶中，约2%在内质网中。与对照孵育相比，用³H - 乙酰化核糖核酸酶孵育相同时间后，成纤维细胞核RNA损失了47%，细胞质溶胶RNA量增加了约40%。在低浓度下孵育3小时期间，具有生物活性的巨噬细胞核糖核酸酶刺激³H - 胸腺嘧啶核苷掺入增殖性肉芽组织切片的DNA中。因此得出结论，巨噬细胞核糖核酸酶对肉芽组织成纤维细胞的促纤维化作用是通过RNA代谢发生的，该酶在成纤维细胞中的主要作用靶点是核RNA。
