School of Pharmacy, Anhui Medical University Department of Pharmacy, The Second Hospital of Anhui Medical University, Hefei, Anhui Province, China.
J Pharm Pharmacol. 2012 Dec;64(12):1751-60. doi: 10.1111/j.2042-7158.2012.01550.x. Epub 2012 Jul 22.
The aim of this study was to investigate the prophylactic effect and some mechanisms of action of triterpene acids of loquat (TAL) on bleomycin A5-induced pulmonary fibrosis rats.
A model of pulmonary fibrosis was induced by injecting rats with a single dose of bleomycin A5 (5 mg/kg) into the trachea. From the second day, rats in the preventive groups were treated with TAL (50, 150 or 450 mg/kg) or dexamethasone (1.2 mg/kg). On the 28th day after medication, the rats were killed and haematoxylin-eosin or masson staining was used to evaluate the degree of pulmonary fibrosis. Tumour necrosis factor-α (TNF-α) and transforming growth factor-β1 (TGF-β1) levels in alveolar macrophage culture supernatant were detected by ELISA. The mRNA expression of TNF-α and TGF-β1 in alveolar macrophage was observed by RT-PCR.
Lung histopathological examination showed TAL could ameliorate the structure of the lung and alleviate fibrogenesis. At the same time, TAL (150 or 450 mg/kg dose group) could reduce the expression of TNF-α and TGF-β1 in alveolar macrophage of rats with pulmonary fibrosis at either the protein or mRNA level.
TAL had a positive prophylactic effect on lung fibrosis, which might have been related to its reduction on TNF-α or TGF-β1 expression in the alveolar macrophage of pulmonary fibrosis rats.
本研究旨在探讨枇杷三萜酸（TAL）对博来霉素 A5 诱导的肺纤维化大鼠的预防作用及其部分作用机制。
通过向大鼠气管内单次注射博来霉素 A5（5mg/kg）建立肺纤维化模型。从第二天开始，预防组大鼠给予 TAL（50、150 或 450mg/kg）或地塞米松（1.2mg/kg）治疗。给药 28 天后，处死大鼠，采用苏木精-伊红或马松染色法评价肺纤维化程度。酶联免疫吸附试验（ELISA）检测肺泡巨噬细胞培养上清液中肿瘤坏死因子-α（TNF-α）和转化生长因子-β1（TGF-β1）的水平。RT-PCR 观察肺泡巨噬细胞中 TNF-α和 TGF-β1 的 mRNA 表达。
肺组织病理学检查结果表明，TAL 可改善肺组织结构，减轻纤维化。同时，TAL（150 或 450mg/kg 剂量组）可降低肺纤维化大鼠肺泡巨噬细胞中 TNF-α和 TGF-β1 的蛋白和 mRNA 表达。
TAL 对肺纤维化具有积极的预防作用，这可能与其降低肺纤维化大鼠肺泡巨噬细胞中 TNF-α或 TGF-β1 的表达有关。
