Cotte Axelle, Foroozandeh Mohammadali, Jeannerat Damien
University of Geneva, Department of organic chemistry, Quai Ernest Ansermet, 30 CH-1211 Genève 4, Switzerland.
Chimia (Aarau). 2012;66(10):764-9. doi: 10.2533/chimia.2012.764.
Broadband homonuclear decoupling (BBHD) in the indirect (1)H dimension of 2D experiments can be obtained using a modified Zangger and Sterk combination of a selective pulse with a pulsed-field gradient. The coupling structure of signals is reduced to a singlet along the F1 dimension at the cost of a sensitivity loss. With the classical sampling in F1, the full resolving power of BBHD-experiments requires very long acquisition times. Spectral aliasing can reduce the number of time increments accessing the top resolution of homodecoupled spectra of small molecules by two orders of magnitude. The TOCSY spectra of androst-4-ene-3,17-dione are shown as an example.
在二维实验的间接氢（¹H）维度中，宽带同核去耦（BBHD）可通过使用选择性脉冲与脉冲场梯度的改良赞格-斯特克组合来实现。信号的耦合结构沿F1维度被简化为单峰，但代价是灵敏度损失。对于F1维度的传统采样，BBHD实验的完整分辨能力需要非常长的采集时间。光谱混叠会使能够达到小分子同核去耦光谱最高分辨率的时间增量数量减少两个数量级。以雄甾-4-烯-3,17-二酮的TOCSY光谱为例进行展示。
