Public Health Ontario, Toronto, ON, Canada.
Epidemiol Infect. 2013 Aug;141(8):1781-5. doi: 10.1017/S0950268812002531. Epub 2012 Nov 12.
The objective of this study was to determine the optimal number of respiratory samples per outbreak to be tested for institutional respiratory outbreaks in Ontario. We reviewed respiratory samples tested for respiratory viruses by multiplex PCR as part of outbreak investigations. We documented outbreaks that were positive for any respiratory viruses and for influenza alone. At least one virus was detected in 1454 (85∙2%) outbreaks. The ability to detect influenza or any respiratory virus increased as the number of samples tested increased. When analysed by chronological order of when samples were received at the laboratory, percent positivity of outbreaks testing positive for any respiratory virus including influenza increased with the number of samples tested up to the ninth sample, with minimal benefit beyond the fourth sample tested. Testing up to four respiratory samples per outbreak was sufficient to detect viral organisms and resulted in significant savings for outbreak investigations.
本研究旨在确定安大略省医疗机构呼吸道暴发疫情检测所需的最佳呼吸道样本数量。我们对作为暴发调查一部分而进行的呼吸道病毒多重 PCR 检测的呼吸道样本进行了回顾。我们记录了呼吸道病毒检测阳性的暴发疫情，包括任何呼吸道病毒和流感病毒单独阳性的情况。在 1454 次暴发疫情（85.2%）中至少检测到一种病毒。随着检测样本数量的增加，检测流感或任何呼吸道病毒的能力均有所提高。按照实验室收到样本的时间顺序进行分析时，检测任何呼吸道病毒（包括流感）阳性的暴发疫情阳性率随着检测样本数量的增加而增加，直到第 9 个样本，第 4 个样本之后则获益甚微。每个暴发疫情检测 4 个呼吸道样本足以检测到病毒病原体，这为暴发疫情调查节省了大量成本。
