Carlton S M, Westlund K N, Zhang D X, Sorkin L S, Willis W D
Department of Anatomy and Neurosciences, University of Texas Medical Branch, Galveston 77550.
Neurosci Lett. 1990 Feb 5;109(1-2):76-81. doi: 10.1016/0304-3940(90)90540-p.
Spinothalamic tract (STT) cells were identified by intracellular injection or retrograde labeling with horseradish peroxidase (HRP) in the primate spinal cord. Using immunohistochemical techniques, a population of primary afferents containing calcitonin gene-related peptide (CGRP) is demonstrated in synaptic contact with these neurons. Large glomerular type CGRP terminals with morphology considered typical of primary afferent fibers are occasionally observed in contact with STT profiles in laminae I and IIo; however, this morphological type targets STT profiles chiefly in the deeper dorsal horn (laminae III, IV and V). In contrast, the majority of CGRP terminals contacting STT profiles in the superficial dorsal horn (laminae I and IIo), are small, round or oblong shaped terminals. Thus, evidenced by these data, the absence of large, glomerular type terminals does not rule out primary afferent input.
在灵长类动物脊髓中，通过细胞内注射或用辣根过氧化物酶（HRP）逆行标记来鉴定脊髓丘脑束（STT）细胞。运用免疫组织化学技术，可证明一群含有降钙素基因相关肽（CGRP）的初级传入神经与这些神经元形成突触联系。偶尔会在板层I和IIo中观察到与STT轮廓相接触的、具有典型初级传入纤维形态的大型肾小球型CGRP终末；然而，这种形态类型的主要靶点是背角深层（板层III、IV和V）的STT轮廓。相比之下，在背角浅层（板层I和IIo）与STT轮廓相接触的大多数CGRP终末是小的圆形或椭圆形终末。因此，这些数据表明，大型肾小球型终末的缺失并不排除初级传入神经的输入。
