Ogawa Hirohito, Chahota Rajesh, Ohya Kenji, Yamaguchi Tsuyoshi, Fukushi Hideto
Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu, Japan.
J Vet Med Sci. 2013 May 2;75(4):503-7. doi: 10.1292/jvms.12-0367. Epub 2012 Nov 12.
Beak and feather disease virus (BFDV) is a causative agent of psittacine beak and feather disease (PBFD), which shows a characteristic feather disorder in psittacine birds. In the present study, the subclinical infection rate of PBFD in imported and domestically bred psittacine birds was investigated by polymerase chain reaction. As a result, 126 of 402 birds (31.3%) were found to be BFDV positive. The DNA sequences of the part of open reading frame (ORF) C1 were determined for 16 BFDV-positive randomly selected samples. One of 16 samples was found to have a mixed infection, and 5 different BFDV sequences were obtained from a single African grey parrot. In phylogenic analysis, almost BFDV sequences included in each genetic cluster of phylogenic tree belonged to the same psittacine subfamily. BFDV derived from African grey parrot was closely related to the BFDV derived from cockatoos by way of exception. The natural habitat of the African grey parrot and cockatoos is different, and therefore, the possibility of interspecies cross infection through the bird trade is suggested from the exceptional BFDV sequences.
喙羽病病毒（BFDV）是鹦鹉喙羽病（PBFD）的病原体，该病在鹦鹉类鸟类中表现出特征性的羽毛紊乱。在本研究中，通过聚合酶链反应对进口和国产鹦鹉类鸟类中PBFD的亚临床感染率进行了调查。结果发现，402只鸟中有126只（31.3%）BFDV呈阳性。对随机选择的16个BFDV阳性样本测定了开放阅读框（ORF）C1部分的DNA序列。16个样本中有1个发现有混合感染，并且从一只非洲灰鹦鹉中获得了5种不同的BFDV序列。在系统发育分析中，系统发育树各遗传簇中包含的几乎所有BFDV序列都属于同一鹦鹉亚科。例外的是，源自非洲灰鹦鹉的BFDV与源自凤头鹦鹉的BFDV密切相关。非洲灰鹦鹉和凤头鹦鹉的自然栖息地不同，因此，从这些例外的BFDV序列推测存在通过鸟类贸易进行种间交叉感染的可能性。
