Ogawa Hirohito, Katoh Hiroshi, Sanada Naoko, Sanada Yasuyuki, Ohya Kenji, Yamaguchi Tsuyoshi, Fukushi Hideto
Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, Gifu, Gifu, Japan.
Virus Genes. 2010 Oct;41(2):231-5. doi: 10.1007/s11262-010-0509-0. Epub 2010 Jul 25.
Beak and feather disease virus (BFDV) is a causative agent for psittacine beak and feather disease (PBFD), which shows a characteristic feather disorder in psittacine birds. Nineteen budgerigars, which were clinically suspected to have PBFD, were examined by two polymerase chain reactions (PCR), which target each of open reading frames (ORFs) V1 and C1. All of the 19 samples were detected BFDV by the PCR targeting ORF C1, whereas only two of them were detected by the PCR targeting ORF V1. It was assumed that BFDV derived from budgerigar (budgerigar BFDV) has two genotypes, which are tentatively classified as budgerigar BFDV genotype 1 and genotype 2 by the PCR amplification patterns. Whole genome sequences of six budgerigar BFDVs were determined to reveal the existence of two genotypes. In the phylogenic analysis, six budgerigar BFDV sequences formed a unique group branched from the other 23 published BFDV sequences. The budgerigar BFDV genotype 1 and genotype 2 were also segregated each other, and budgerigar BFDV genotype 2 was particularly distantly related with the other BFDVs. These results suggest budgerigar BFDV is a unique in the known BFDVs and is divided into two genotypes.
喙羽病病毒（BFDV）是鹦鹉喙羽病（PBFD）的病原体，该病在鹦鹉类鸟类中表现出特征性的羽毛紊乱症状。对19只临床疑似患有PBFD的虎皮鹦鹉进行了两项聚合酶链反应（PCR）检测，这两项检测分别针对开放阅读框（ORF）V1和C1。通过针对ORF C1的PCR检测，19个样本均检测到BFDV，而通过针对ORF V1的PCR检测，仅其中两个样本检测到BFDV。据推测，源自虎皮鹦鹉的BFDV（虎皮鹦鹉BFDV）有两种基因型，根据PCR扩增模式暂分为虎皮鹦鹉BFDV基因型1和基因型2。测定了6株虎皮鹦鹉BFDV的全基因组序列，以揭示两种基因型的存在。在系统发育分析中，6条虎皮鹦鹉BFDV序列形成了一个独特的分支，与其他23条已发表的BFDV序列分支开来。虎皮鹦鹉BFDV基因型1和基因型2也相互分离，其中虎皮鹦鹉BFDV基因型2与其他BFDV的亲缘关系尤为疏远。这些结果表明，虎皮鹦鹉BFDV在已知的BFDV中是独特的，并且分为两种基因型。
