用于视网膜变性和疾病研究的小鼠模型。
Mouse models for studies of retinal degeneration and diseases.
作者信息
Chang Bo
机构信息
The Jackson Laboratory, Bar Harbor, ME, USA,
出版信息
Methods Mol Biol. 2013;935:27-39. doi: 10.1007/978-1-62703-080-9_2.
Abstract
Mouse models, with their well-developed genetics and similarity to human physiology and anatomy, serve as powerful tools with which to investigate the etiology of human retinal degeneration. Mutant mice also provide reproducible, experimental systems for elucidating pathways of normal development and function. Here, I describe the tools used in the discoveries of many retinal degeneration models, including indirect ophthalmoscopy (to look at the fundus appearance), fundus photography and fluorescein angiography (to document the fundus appearance), electroretinography (to check retinal function), as well as the heritability test (for genetic characterization).
摘要
小鼠模型因其完善的遗传学以及与人类生理和解剖学的相似性，成为研究人类视网膜变性病因的有力工具。突变小鼠还为阐明正常发育和功能途径提供了可重复的实验系统。在此，我描述了在许多视网膜变性模型发现过程中所使用的工具，包括间接检眼镜检查（用于观察眼底外观）、眼底照相和荧光素血管造影（用于记录眼底外观）、视网膜电图（用于检查视网膜功能）以及遗传性测试（用于基因特征分析）。
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