Segal Gil
George S. Wise Faculty of Life Sciences, Department of Molecular MIcrobiology and Biotechnology, Tel-Aviv University, Ramat-Aviv, Israel.
Methods Mol Biol. 2013;954:595-602. doi: 10.1007/978-1-62703-161-5_37.
Legionella pneumophila the causative agent of Legionnaires' disease, actively manipulates host cell processes to establish a replication niche inside host cells. The establishment of its replication niche requires a functional Icm/Dot type IV secretion system which translocates about 300 effector proteins into host cells during infection. Many of these effectors were first identified as effector candidates by several bioinformatic approaches, and these predicted effectors were later examined experimentally for translocation and a large number of which were validated as effector proteins. Here, I summarized the bioinformatic approaches that were used to identify these effectors.
嗜肺军团菌是军团病的病原体，它会积极操控宿主细胞进程，以便在宿主细胞内建立一个复制龛。其复制龛的建立需要一个功能性的Icm/Dot IV型分泌系统，该系统在感染过程中将约300种效应蛋白转运到宿主细胞中。这些效应蛋白中有许多最初是通过几种生物信息学方法被鉴定为效应蛋白候选物的，随后对这些预测的效应蛋白进行了转运实验检测，其中大量被确认为效应蛋白。在此，我总结了用于鉴定这些效应蛋白的生物信息学方法。
