Department of Chemistry, Bard College, 30 Campus Rd, Annandale-on-Hudson, New York 12504, United States.
Inorg Chem. 2012 Dec 3;51(23):12917-24. doi: 10.1021/ic301981s. Epub 2012 Nov 14.
Hetero-multinuclear, platinum/ruthenium species were synthesized and tested for their effect on the motility of A549 (nonsmall cell lung) and MDA-MB-231 (breast) cancer cells and for their ability to inhibit DNA mobility using gel electrophoresis. It was found that the Ru(2)Pt trinuclear species [Na(2)]{Ru(III)Cl(4)(DMSO-S)(-μ-pyz)Pt(II)Cl(2)}, AH197, was much more efficient at inhibiting cell motility than [C(3)N(2)H(5)][Ru(III)Cl(4)(DMSO-S)(C(3)N(2)H(4))], NAMI-A, while the dinuclear RuPt species [K][Ru(III)Cl(4)(DMSO-S)(-μ-pyz)Pt(II)(DMSO-S)Cl(2)], IT127, was slightly better than NAMI-A. However, the dinuclear species retarded the electrophoretic mobility of DNA greater than both the trinuclear complex and cisplatin. The metal complexes and their respective BSA protein/metal adducts were studied by X-ray absorption spectroscopy. The spectra led to the conclusion that BSA donor atoms have substituted for the chloride ligands and perhaps the DMSO ligands.
合成了杂多核铂/钌配合物，并研究了它们对 A549（非小细胞肺癌）和 MDA-MB-231（乳腺癌）癌细胞迁移的影响，以及用凝胶电泳抑制 DNA 迁移的能力。结果发现，Ru(2)Pt 三核物种 [Na(2)]{Ru(III)Cl(4)(DMSO-S)(-μ-pyz)Pt(II)Cl(2)}，AH197，比 [C(3)N(2)H(5)][Ru(III)Cl(4)(DMSO-S)(C(3)N(2)H(4))]，NAMI-A，更有效地抑制细胞迁移，而双核 RuPt 物种 [K][Ru(III)Cl(4)(DMSO-S)(-μ-pyz)Pt(II)(DMSO-S)Cl(2)]，IT127，略优于 NAMI-A。然而，双核物种使 DNA 的电泳迁移率比三核配合物和顺铂都慢。通过 X 射线吸收光谱研究了金属配合物及其相应的 BSA 蛋白/金属加合物。光谱得出的结论是，BSA 的供体原子取代了氯配体，也许还取代了 DMSO 配体。
