Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.
Foodborne Pathog Dis. 2013 Jan;10(1):21-7. doi: 10.1089/fpd.2012.1247. Epub 2012 Nov 15.
Yersinia pseudotuberculosis is a foodborne pathogen often detected and identified using polymerase chain reaction (PCR) with primers targeted to virulence genes. Sequence variability of the virulence genes in strains representing different serotypes is unknown. Sequence variability could hinder the recognition of this pathogen by PCR and affect the host-pathogen interactions. Sequencing of inv, virF, and yadA of 18 Y. pseudotuberculosis strains showed limited variability of inv and virF, whereas the sequences of yadA varied considerably.
类鼻疽假单胞菌是一种食源性病原体，通常使用针对毒力基因的聚合酶链反应 (PCR) 进行检测和鉴定。不同血清型菌株的毒力基因序列变异情况尚不清楚。序列变异可能会阻碍 PCR 对该病原体的识别，并影响宿主-病原体相互作用。对 18 株类鼻疽假单胞菌的 inv、virF 和 yadA 进行测序显示，inv 和 virF 的序列变异有限，而 yadA 的序列则有较大差异。
