基于环介导等温扩增技术对假结核耶尔森菌进行灵敏且特异的检测
Sensitive and specific detection of Yersinia pseudotuberculosis by loop-mediated isothermal amplification.
作者信息
Horisaka Tomoko, Fujita Kayoko, Iwata Taketoshi, Nakadai Aya, Okatani Alexandre T, Horikita Tetsuya, Taniguchi Takahide, Honda Eiichi, Yokomizo Yuichi, Hayashidani Hideki
机构信息
Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
出版信息
J Clin Microbiol. 2004 Nov;42(11):5349-52. doi: 10.1128/JCM.42.11.5349-5352.2004.
Abstract
We developed a loop-mediated isothermal amplification method able to detect Yersinia pseudotuberculosis strains in 30 min by using six primers designed by targeting the inv gene. This method is more sensitive than PCR and might be a useful tool for detecting and identifying Y. pseudotuberculosis.
摘要
我们开发了一种环介导等温扩增方法，通过使用针对inv基因设计的六种引物，能够在30分钟内检测出假结核耶尔森菌菌株。该方法比聚合酶链反应（PCR）更灵敏，可能是检测和鉴定假结核耶尔森菌的有用工具。
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Sensitive and specific detection of Yersinia pseudotuberculosis by loop-mediated isothermal amplification.基于环介导等温扩增技术对假结核耶尔森菌进行灵敏且特异的检测
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