College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Daehak-Dong, Gwanak-Gu, Seoul, Republic of Korea.
Planta Med. 2013 Jan;79(1):78-82. doi: 10.1055/s-0032-1327949. Epub 2012 Nov 15.
Bioassay-guided fractionation of an 80% methanolic extract of Salix pseudo-lasiogyne twigs has resulted in the isolation of two new compounds (1-2) along with ten known ones (3-12). The new compounds were determined to be 3'-O-acetylsalicin (1) and 2',6'-O-acetylsalicortin (2) by using spectroscopic analyses. Compounds (3-12) were identified as salicin (3), 2'-O-acetylsalicin (4), salicortin (5), 2'-O-acetylsalicortin (6), 3'-O-acetylsalicortin (7), 6'-O-acetylsalicortin (8), 2'-O-(E)-ρ-coumaroylsalicortin (9), grandidentatin (10), isograndidentatin (11), and saligenin (12). Among the isolated compounds, compounds 2, 5, 6, 7, and 8 bearing 1-hydroxy-6-oxo-2-cyclohexenecarboxylate moiety significantly inhibited lipopolysaccharide-induced nitric oxide production in BV2 microglial cells in vitro. Further, we studied anti-amnesic activities of the 80% methanolic extract, the EtOAc fraction, and compound 6 from S. pseudo-lasiogyne. They exerted a significant cognitive-enhancing effect on scopolamine-induced memory deficit in mice. In addition, they also significantly increased the reduced activities of glutathione reductase and superoxide dismutase and the glutathione content in the hippocampus and cortex of scopolamine-induced amnesic mice.
采用生物测定指导的方法，对柳属植物 pseudo-lasiogyne 小枝的 80%甲醇提取物进行分段分离，得到了两种新化合物（1-2）和十种已知化合物（3-12）。通过光谱分析，确定这两种新化合物分别为 3'-O-乙酰水杨苷（1）和 2',6'-O-乙酰水杨苷（2）。化合物（3-12）分别被鉴定为水杨苷（3）、2'-O-乙酰水杨苷（4）、水杨苷（5）、2'-O-乙酰水杨苷（6）、3'-O-乙酰水杨苷（7）、6'-O-乙酰水杨苷（8）、2'-O-（E）-对香豆酰水杨苷（9）、大柳杉酚（10）、异大柳杉酚（11）和水杨醇（12）。在所分离的化合物中，具有 1-羟基-6-氧代-2-环己烯羧酸酯部分的化合物 2、5、6、7 和 8 显著抑制了 LPS 诱导的 BV2 小胶质细胞体外一氧化氮的产生。此外，我们研究了 pseudo-lasiogyne 80%甲醇提取物、EtOAc 馏分和化合物 6 的抗健忘活性。它们对东莨菪碱诱导的小鼠记忆缺陷有显著的认知增强作用。此外，它们还显著增加了东莨菪碱诱导的健忘小鼠海马和皮质中谷胱甘肽还原酶、超氧化物歧化酶和谷胱甘肽的活性。
