Department of Orthopaedics, Wannan Medical College, Wuhu, Anhui, China.
Chin Med J (Engl). 2012 Nov;125(22):4055-60.
This study aimed to investigate the effect of pcDNA3.1-vascular endothelial growth factor (VEGF)165 vector on vertebral cartilage endplate vascular buds and intervertebral discs.
Rabbits were randomly assigned to the control and experimental groups with 10 in each. In the experimental group, we anesthetized the rabbits and exposed the front vertebral body. Using the mark of the longitudinal ossature of the front vertebral body of the lumbar vertebrae, we advanced a needle at the central point of the front fourth and fifth lumbar intervertebral discs and injected 20 µl pcDNA3.1-VEGF165. Similarly, in the control group, we injected 20 µl pcDNA3.1. At 4 and 8 weeks post-injection, we examined the changes of the vertebral cartilage endplate using X-ray radiograph, histology, and scanning electron microscopy.
The vertebral cartilage endplate calcification and degeneration in the experimental group were less than those in the control group at 8 weeks post-operation. The average number and diameter of vascular buds obviously increased in the experimental group at 4 and 8 weeks post-operation. The number of vascular buds and the diameter in the region of the inner annulus increased when compared to those in the area near the nucleus pulposus.
The pcDNA3.1-VEGF165 plasmid can increase the average number and diameter of vascular buds and decelerate intervertebral disc degeneration.
本研究旨在探讨 pcDNA3.1-血管内皮生长因子（VEGF）165 载体对椎体软骨终板血管芽和椎间盘的影响。
将兔子随机分为对照组和实验组，每组 10 只。实验组麻醉兔子，暴露前椎体。在前腰椎体的前纵骨标志处，在前第四和第五腰椎间盘的中心点进针，注射 20µl pcDNA3.1-VEGF165。同样，在对照组中，我们注射了 20µl pcDNA3.1。在注射后 4 和 8 周，我们使用 X 射线射线照相、组织学和扫描电子显微镜检查椎体软骨终板的变化。
实验组术后 8 周，椎体软骨终板钙化和退变程度低于对照组。实验组术后 4 和 8 周，血管芽的平均数量和直径明显增加。与靠近髓核区域相比，内环区域的血管芽数量和直径增加。
pcDNA3.1-VEGF165 质粒可增加血管芽的平均数量和直径，减缓椎间盘退变。
