[原发性胆汁性肝硬化患者外周血单个核细胞T细胞中的微小RNA谱分析]
[MicroRNA profiling in T cells of peripheral blood mononuclear cell from patients with primary biliary cirrhosis].
作者信息
Qian Cheng, Wang Huai-zhou, Fan Hua-jie, Gu Ming-li, Ren Chuan-lu, Deng An-mei, Zhong Ren-qian
机构信息
Department of Laboratory Medicine, No.100 Hospital of PLA, Suzhou, China.
出版信息
Zhonghua Yi Xue Za Zhi. 2012 Aug 28;92(32):2265-7.
OBJECTIVE
To explore the expression pattern of microRNA (miRNA) in T cells of peripheral blood mononuclear cell (PBMC) from patients with primary biliary cirrhosis (PBC).
METHODS
The expression profile of miRNA in T cells of PBMC was determined by microarray assay and validated by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
RESULTS
In comparison with the healthy controls, 23 miRNA were down-regulated and 2 miRNA had a higher expression (all P < 0.05). As revealed by qRT-PCR, the expressions of miR-346, miR-17-5p, miR-20a and miR-let-7b decreased obviously while miR-451 and miR-129 became up-regulated. The results were in agreement with those of microarray.
CONCLUSIONS
The PBC patients and healthy controls have significantly different expression profiles of microRNA in T cells of PBMC. The differential expression of microRNA may be involved in the pathogenesis of PBC.
目的
探讨原发性胆汁性肝硬化（PBC）患者外周血单个核细胞（PBMC）中T细胞微小RNA（miRNA）的表达模式。
方法
采用基因芯片技术检测PBMC中T细胞miRNA的表达谱，并通过实时定量逆转录聚合酶链反应（qRT-PCR）进行验证。
结果
与健康对照相比，23种miRNA表达下调，2种miRNA表达上调（均P < 0.05）。qRT-PCR结果显示，miR-346、miR-17-5p、miR-20a和miR-let-7b表达明显降低，而miR-451和miR-129表达上调。结果与基因芯片结果一致。
结论
PBC患者与健康对照PBMC中T细胞的miRNA表达谱存在显著差异。miRNA的差异表达可能参与PBC的发病机制。
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