原发性胆汁性肝硬化患者中S100A12的表达
S100A12 expression in patients with primary biliary cirrhosis.
作者信息
Ma Danxu, Li Xi, Zhang Lei, Deng Chuiwen, Zhang Ting, Wang Li, Hu Chaojun, Li Yongzhe, Zhang Fengchun
机构信息
Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences , Beijing , China .
出版信息
Immunol Invest. 2015;44(1):13-22. doi: 10.3109/08820139.2014.914530. Epub 2014 Oct 14.
OBJECTIVES
S100 calcium binding protein A12 (S100A12) has been supposed to be a pro-inflammatory factor associated with non-infectious inflammatory diseases. However, whether S100A12 is involved in the inflammatory process of primary biliary cirrhosis (PBC) has not been shown.
METHODS
The levels of S100A12 mRNA transcripts in peripheral mononuclear blood cells (PBMCs) of 66 Chinese patients with primary biliary cirrhosis (PBC), 62 healthy controls (HC) and 55 chronic hepatitis B (CHB) were measured by qRT-PCR. S100A12 serum concentrations in 34 PBC patients were measured by ELISA.
RESULTS
The levels of S100A12 mRNA transcripts in PBMCs of patients with PBC were significantly higher than healthy controls (p < 0.01) and that of patients with CHB (p < 0.01). Importantly, the levels of S100A12 mRNA in PBMCs and S100A12 protein levels in serum were positively correlated with biochemical indicators of bile duct and hepatocyte damage.
CONCLUSION
S100A12 might participate in the damage of biliary epithelial cells and hepatocytes in PBC, and analysis of S100A12 expression could be useful as a surrogate marker for the evaluation of PBC activity.
目的
S100钙结合蛋白A12（S100A12）被认为是一种与非感染性炎症性疾病相关的促炎因子。然而，S100A12是否参与原发性胆汁性肝硬化（PBC）的炎症过程尚未见报道。
方法
采用qRT-PCR检测66例中国原发性胆汁性肝硬化（PBC）患者、62例健康对照（HC）和55例慢性乙型肝炎（CHB）患者外周血单个核细胞（PBMC）中S100A12 mRNA转录水平。采用ELISA法检测34例PBC患者血清中S100A12浓度。
结果
PBC患者PBMC中S100A12 mRNA转录水平显著高于健康对照（p＜0.01）和CHB患者（p＜0.01）。重要的是，PBMC中S100A12 mRNA水平和血清中S100A12蛋白水平与胆管和肝细胞损伤的生化指标呈正相关。
结论
S100A12可能参与PBC患者胆管上皮细胞和肝细胞的损伤，S100A12表达分析可作为评估PBC活动的替代标志物。
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