Abdel-Rahman Mohamed Nabih, Kabel Ahmed Mohammed
Department of Pharmacology, Faculty of Medicine, Tanta University, Egypt.
J Egypt Natl Canc Inst. 2012 Dec;24(4):161-7. doi: 10.1016/j.jnci.2012.08.001. Epub 2012 Sep 20.
SEC is an undifferentiated tumour used in tumour studies. MTX is an antimetabolite used in treatment of cancer, autoimmune diseases and induction of abortion. VPA is used as anticonvulsant and is under investigation for treatment of cancer. The aim of this work was to compare between the effect of each of MTX and VPA on solid Ehrlich tumour in mice.
Forty albino mice were divided into 4 equal groups: control untreated group, SEC group, SEC+MTX group, and SEC+VPA group. Tumour volume, tissue CAT, tissue GR, tissue MDA, tissue cholesterol and tissue TNF-α were determined. A part of the tumour was examined for histopathological and immunohistochemical study.
MTX alone or VPA alone induced a significant increase in tissue CAT and GR with a significant decrease in the tumour volume, tissue MDA, cholesterol and TNF-α and alleviated the histopathological changes with a significant increase in p53 expression compared to SEC group. This effect was more significant in MTX treated group compared to VPA treated group.
MTX has more antitumour effect than VPA against SEC.
肉瘤样癌（SEC）是一种用于肿瘤研究的未分化肿瘤。甲氨蝶呤（MTX）是一种抗代谢物，用于治疗癌症、自身免疫性疾病和引产。丙戊酸（VPA）用作抗惊厥药，目前正在研究其对癌症的治疗作用。本研究的目的是比较MTX和VPA对小鼠实体艾氏腹水癌的影响。
将40只白化小鼠分为4组，每组10只：未治疗的对照组、SEC组、SEC+MTX组和SEC+VPA组。测定肿瘤体积、组织过氧化氢酶（CAT）、组织谷胱甘肽还原酶（GR）、组织丙二醛（MDA）、组织胆固醇和组织肿瘤坏死因子-α（TNF-α）。取部分肿瘤组织进行组织病理学和免疫组织化学研究。
与SEC组相比，单独使用MTX或VPA均可显著提高组织CAT和GR水平，显著降低肿瘤体积、组织MDA、胆固醇和TNF-α水平，并减轻组织病理学变化，p53表达显著增加。与VPA治疗组相比，MTX治疗组的这种效果更显著。
MTX对SEC的抗肿瘤作用比VPA更强。
