Duke University Medical Center, Department of Medicine, Durham, NC 27705, USA.
Clin Immunol. 2013 Jan;146(1):1-9. doi: 10.1016/j.clim.2012.10.004. Epub 2012 Oct 13.
Systemic lupus erythematosus is a systemic inflammatory disease characterized by antibodies to nuclear molecules in association with immune complex deposition. As shown previously, microparticles (MPs), which are small membrane-bound vesicles released from dying and activated cells, contain nucleic acids and can form immune complexes found in patient blood. To assess the role of MPs in murine lupus, we used flow cytometry to measure the presence of MPs with bound IgG in the blood of MRL-lpr/lpr and NZB/W mice. These studies showed much higher numbers of MPs with bound IgG in the blood of MRL lpr/lpr compared to NZB/W mice. Furthermore, these studies showed that antibodies from MRL-lpr/lpr mice bound better to MPs from apoptotic cells than those from NZB/W mice. Together, these studies indicate important differences in the serological features of the two strains as reflected by the capacity of antibodies to bind to MPs.
系统性红斑狼疮是一种全身性炎症性疾病，其特征是存在针对核分子的抗体，并伴有免疫复合物沉积。如前所述，微颗粒（MPs）是从死亡和激活的细胞中释放的小膜结合囊泡，含有核酸，并可形成在患者血液中发现的免疫复合物。为了评估 MPs 在鼠狼疮中的作用，我们使用流式细胞术测量了 MRL-lpr/lpr 和 NZB/W 小鼠血液中结合 IgG 的 MPs 的存在。这些研究表明，与 NZB/W 小鼠相比，MRL lpr/lpr 血液中的结合 IgG 的 MPs 数量要高得多。此外，这些研究表明，来自 MRL-lpr/lpr 小鼠的抗体与来自凋亡细胞的 MPs 的结合能力优于来自 NZB/W 小鼠的抗体。综上所述，这些研究表明，两种品系在血清学特征上存在重要差异，这反映在抗体与 MPs 的结合能力上。
