College of Animal Science/Guangdong Provincial Key Lab of Agro-animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou, Guangdong, 510642, People's Republic of China.
Cell Signal. 2013 Feb;25(2):561-9. doi: 10.1016/j.cellsig.2012.11.003. Epub 2012 Nov 13.
Notch signaling is an evolutionarily conserved cell-cell communication mechanism involved in the regulation of cell proliferation, differentiation and fate decisions of mammalian cells. In the present study, we investigated the possible requirement for Notch signaling in the proliferation and differentiation of porcine satellite cells. We show that Notch1, 2 and 3 are expressed in cultured porcine satellite cells. Knock-down of NOTCH1, but not NOTCH2 and NOTCH3, decreases the proliferation of porcine satellite cells. In contrast, enhancement of NOTCH1 expression via treatment of porcine satellite cells with recombinant NF-κB increases the proliferation of porcine satellite cells. The alteration of porcine satellite cell proliferation is associated with significant changes in the expression of cell cycle related genes (cyclin B1, D1, D2, E1 and p21), myogenic regulatory factors (MyoD and myogenin) and the Notch effector Hes5. In addition, alteration of Notch1 expression in porcine satellite cells causes changes in the expression of GSK3β-3. Taken together, these findings suggest that of the four notch-related genes, Notch1is likely to be required for regulating the proliferation and therefore the maintenance of porcine satellite cells in vivo, and do so through activation of the Notch effector gene Hes5.
Notch 信号通路是一种进化上保守的细胞间通讯机制，参与哺乳动物细胞的增殖、分化和命运决定。在本研究中，我们研究了 Notch 信号通路在猪卫星细胞增殖和分化中的可能作用。研究结果表明，Notch1、2 和 3 在培养的猪卫星细胞中表达。Notch1 的敲低而非 Notch2 和 Notch3 的敲低可降低猪卫星细胞的增殖。相反，通过用重组 NF-κB 处理猪卫星细胞增强 Notch1 的表达可增加猪卫星细胞的增殖。猪卫星细胞增殖的改变与细胞周期相关基因（cyclin B1、D1、D2、E1 和 p21）、成肌调节因子（MyoD 和 myogenin）和 Notch 效应因子 Hes5 的表达显著变化相关。此外，猪卫星细胞中 Notch1 表达的改变导致 GSK3β-3 的表达改变。综上所述，这些发现表明，在这四个 Notch 相关基因中，Notch1 可能是调节猪卫星细胞在体内增殖和维持的关键基因，其通过激活 Notch 效应基因 Hes5 发挥作用。
