Department of Pathology, University of Ioannina, Ioannina, Greece.
In Vivo. 2012 Nov-Dec;26(6):985-92.
Dendritic cells play key roles in thymic histophysiology and histopathology. Therefore, we analyzed the immunotopographical distribution of cells expressing markers of dendritic cells and macrophages in postnatal human thymus.
The streptavidin-biotin peroxidise-labeled (LSAB) and the double-LSAB/alkaline phosphatase/anti-alkaline phosphatase (APAAP) immunohistochemical procedures were used.
S100 protein-, Cluster of designation 1a (CD1a)-, CD207-, CD11c- and CD123-positive cells, many of them exhibiting the morphology of dendritic cells, were detected in the cortex but mainly in the medulla. These markers, except CD123, were also detected in cells of juvenile and immature Hassall bodies. CD68- and CD163-positive cells were detected in the cortex and the medulla but not in Hassall bodies.
The immunohistological detection of S100-, CD1a-, CD207- and CD11c-positive dendritic cells in juvenile and immature Hassall bodies may reflect an important role of these structures in the cooperation of epithelial and dendritic cells in the process of T-cell differentiation.
树突状细胞在胸腺组织学和组织病理学中发挥关键作用。因此，我们分析了在人胸腺后天中表达树突状细胞和巨噬细胞标志物的细胞的免疫拓扑分布。
采用链霉亲和素-生物素过氧化物酶标记（LSAB）和双 LSAB/碱性磷酸酶/抗碱性磷酸酶（APAAP）免疫组织化学方法。
在皮质中但主要在髓质中检测到 S100 蛋白、分化簇 1a（CD1a）、CD207、CD11c 和 CD123 阳性细胞，其中许多细胞呈现树突状细胞的形态。这些标记物，除了 CD123，也在青少年和未成熟的哈索尔体细胞中检测到。CD68 和 CD163 阳性细胞在皮质和髓质中检测到，但在哈索尔体中未检测到。
在青少年和未成熟的哈索尔体中检测到 S100、CD1a、CD207 和 CD11c 阳性树突状细胞，可能反映了这些结构在 T 细胞分化过程中上皮细胞和树突状细胞之间合作中的重要作用。
