Department of Neurology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, People's Republic of China.
J Mol Histol. 2013 Apr;44(2):135-45. doi: 10.1007/s10735-012-9467-2. Epub 2012 Nov 17.
CBLL1 (Casitas B-lineage lymphoma-transforming sequence-like protein 1) also known as Hakai, was originally identified as an E3 ubiquitin-ligase for the E-cadherin complex. Recent data have provided evidences for novel biological functional role of CBLL1 during tumor progression and other diseases. However, its distribution and function in the central nervous system (CNS) remains unclear. In this study, we found CBLL1 was significant up-regulation in cerebral cortex after LPS administration and immunofluorescent labeling indicated that CBLL1 was localized striking in the neurons. We also investigated co-staining of CBLL1 and active-caspase-3 and cyclin D1 in the cerebral cortex following LPS administration. Based on our data, we speculated that CBLL1 might play an important role in neuronal apoptosis following LPS administration and might provide a basis for the further study on its role in cell cycle re-entry in neuroinflammation in CNS.
CBLL1（Casitas B 细胞淋巴瘤转化序列样蛋白 1），也称为 Hakai，最初被鉴定为 E-钙黏蛋白复合物的 E3 泛素连接酶。最近的数据为 CBLL1 在肿瘤进展和其他疾病中的新的生物学功能作用提供了证据。然而，其在中枢神经系统（CNS）中的分布和功能尚不清楚。在这项研究中，我们发现 LPS 给药后大脑皮层中 CBLL1 的表达显著上调，免疫荧光标记表明 CBLL1 特异性定位于神经元中。我们还研究了 LPS 给药后大脑皮层中 CBLL1 与活性-caspase-3 和 cyclin D1 的共染色。基于我们的数据，我们推测 CBLL1 可能在 LPS 给药后的神经元凋亡中发挥重要作用，并为进一步研究其在中枢神经系统神经炎症中细胞周期再进入中的作用提供依据。
