Zhang Yu, Liu Xiaojuan, Xue Huaqing, Liu Xiaorong, Dai Aihua, Song Yan, Ke Kaifu, Cao Maohong
Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
J Mol Neurosci. 2016 May;59(1):146-57. doi: 10.1007/s12031-016-0744-5. Epub 2016 Apr 13.
PRDM5 (PR domain containing 5) belongs to PRDM family which consists of transcriptional regulators that modulate cellular processes such as cell growth, differentiation and apoptosis. However, the function of PRDM5 in central nervous system (CNS) inflammatory response is unknown. In recent study, an adult rat neuroinflammation model via lipopolysaccharide (LPS) lateral ventricle injection was constructed. PRDM5 expression was increased in activated astrocytes and apoptotic neurons of the adult rat cerebral cortex after LPS injection. In vitro studies showed that the remarkable upregulation of PRDM5 might be involved in rat primary astrocyte proliferation and rat primary neuronal apoptosis in the cerebral cortex following LPS administration. In addition, using PRDM5 RNA interference both in rat primary asrtocytes and neurons, further indicated that PRDM5 was required for astrocyte proliferation and neuronal apoptosis induced by LPS. Our findings on the cellular signaling pathway may provide a new therapeutic strategy against neuroinflammation in the CNS.
PRDM5（含PR结构域蛋白5）属于PRDM家族，该家族由调节细胞生长、分化和凋亡等细胞过程的转录调节因子组成。然而，PRDM5在中枢神经系统（CNS）炎症反应中的功能尚不清楚。在最近的研究中，通过脂多糖（LPS）侧脑室注射构建了成年大鼠神经炎症模型。LPS注射后，成年大鼠大脑皮质中活化的星形胶质细胞和凋亡神经元中的PRDM5表达增加。体外研究表明，PRDM5的显著上调可能参与LPS给药后大鼠大脑皮质原代星形胶质细胞增殖和大鼠原代神经元凋亡。此外，在大鼠原代星形胶质细胞和神经元中使用PRDM5 RNA干扰，进一步表明PRDM5是LPS诱导的星形胶质细胞增殖和神经元凋亡所必需的。我们在细胞信号通路方面的研究结果可能为中枢神经系统神经炎症提供一种新的治疗策略。
