Tang Qing, He Xiaohong, Liao Haixing, He Liantu, Wang Ying, Zhou Dazhi, Ye Sumin, Chen Qilu
Department of Sonography, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou, Guangdong 510120;
Oncol Lett. 2012 Nov;4(5):970-972. doi: 10.3892/ol.2012.845. Epub 2012 Aug 6.
The aim of this study was to investigate the role of ultrasound microbubble contrast agent-mediated suicide gene transfection in the treatment of hepatic cancer. We intratumorally injected KDR-TK, AFP-TK and microbubble contrast agent into nude mice prior to ultrasound treatment and administration of prodrugs (GCV and 5-FC). The tumor volume, tumor inhibition rate, survival time and apoptosis of tumor cells was determined. The sizes of subcutaneous hepatic cancers in mice receiving treatment were comparable to those in the control group, and the survival time was similar between the two groups (P>0.05). However, the tumor inhibition rate and the number of apoptotic cells in the treatment group was markedly higher compared with that in the control group (P<0.05). Evident tumor necrosis was absent in both groups, except at the needle tract. Ultrasound therapy following injection of suicide genes and microbubble contrast agents is able to inhibit cancer growth in vivo. This may be attributed to the induction of cancer cell apoptosis.
本研究旨在探讨超声微泡造影剂介导的自杀基因转染在肝癌治疗中的作用。在超声治疗及给予前体药物（GCV和5-FC）之前，我们将KDR-TK、AFP-TK和微泡造影剂瘤内注射到裸鼠体内。测定肿瘤体积、肿瘤抑制率、生存时间及肿瘤细胞凋亡情况。接受治疗的小鼠皮下肝癌大小与对照组相当，两组生存时间相似（P>0.05）。然而，治疗组的肿瘤抑制率和凋亡细胞数量明显高于对照组（P<0.05）。除针道外，两组均未见明显肿瘤坏死。注射自杀基因和微泡造影剂后进行超声治疗能够在体内抑制肿瘤生长。这可能归因于诱导癌细胞凋亡。
