San Francisco VA Medical Center, 4150 Clement Street Box 112, San Francisco, CA 94121, USA.
Regen Med. 2012 Nov;7(6):799-806. doi: 10.2217/rme.12.87.
Current synthetic tubular conduits are inferior to nerve autograft for the repair of segmental peripheral nerve injuries. We examined motor outcomes with the use of longitudinally aligned poly (L-lactide-co-caprolactone) nanofiber conduits for repair of nerve gap injury in a rat model.
Ten-millimeter segments of sciatic nerve were resected in 44 Lewis rats. The gaps were either left unrepaired (n = 6), repaired with nerve autograft (n = 19), or repaired with conduit (n = 19). After 12 weeks, nerve conduction latency, compound muscle action potential amplitude, muscle force and muscle mass were measured. The numbers of axons and axon diameters both within the grafts and distally were determined.
After 12 weeks, gastrocnemius isometric tetanic force and muscle mass for the conduit group reached 85 and 82% of autograft values, respectively. Nerve conduction and compound muscle action potential were not significantly different between these two groups, although the latter approached significance. There was no recovery in the unrepaired group.
Muscle recovery for the animals treated with this aligned nanofiber conduit approached that of autograft, suggesting the importance of internal conduit structure for nerve repair.
目前的合成管状导管在修复节段性周围神经损伤方面不如神经自体移植物。我们使用纵向排列的聚（L-丙交酯-共-己内酯）纳米纤维导管修复大鼠模型中的神经间隙损伤，检查运动结果。
在 44 只 Lewis 大鼠中切除 10 毫米长的坐骨神经段。间隙未修复（n = 6）、用神经自体移植物修复（n = 19）或用导管修复（n = 19）。12 周后，测量神经传导潜伏期、复合肌肉动作电位幅度、肌肉力量和肌肉质量。测量移植物内和远端的轴突数量和轴突直径。
12 周后，导管组的比目鱼肌等长抽搐力和肌肉质量分别达到自体移植物的 85%和 82%。尽管后者接近显著性，但两组之间的神经传导和复合肌肉动作电位没有显著差异。未修复组没有恢复。
用这种排列的纳米纤维导管治疗的动物的肌肉恢复接近自体移植物，这表明内部导管结构对神经修复很重要。
