Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Res Vet Sci. 2013 Jun;94(3):526-30. doi: 10.1016/j.rvsc.2012.10.003. Epub 2012 Nov 16.
Haptoglobin (Hp) is one of the acute phase proteins (APPs) that help to alleviate the immune oxidative damage. The present study expressed a truncated porcine Hp in Escherichia coli and produced rabbit and mouse antisera to the recombinant protein, in order to investigate Hp levels in sera from piglets infected with porcine reproduction and respiratory syndrome virus (PRRSV). Antisera prepared revealed both chains of porcine Hp in Western blot, and mouse antisera showed stronger binding activities than the rabbit antisera. With the combination of Hp monoclonal antibodies, this study confirmed that serum Hp was increased in piglets infected with PRRSV and offered a tool to know about subunit levels of Hp in porcine serum. But Hp itself could not be used as a specific biomarker for PRRSV infection, for elevated Hp levels were also obtained from pigs infected with other pathogens.
触珠蛋白（Hp）是有助于减轻免疫氧化损伤的急性时相蛋白（APP）之一。本研究在大肠杆菌中表达了截断的猪 Hp，并制备了针对重组蛋白的兔和鼠抗血清，以研究感染猪繁殖与呼吸综合征病毒（PRRSV）的仔猪血清中的 Hp 水平。Western blot 分析显示抗血清中存在猪 Hp 的两条链，且鼠抗血清的结合活性强于兔抗血清。结合 Hp 单克隆抗体，本研究证实 PRRSV 感染仔猪的血清 Hp 增加，并提供了一种了解猪血清中 Hp 亚基水平的工具。但 Hp 本身不能作为 PRRSV 感染的特异性生物标志物，因为感染其他病原体的猪也会出现 Hp 水平升高的情况。
