Division of Veterinary Microbiology & Virology, College of Veterinary Medicine, Northwest A & F University, Yangling 712100, China.
Vet Microbiol. 2012 Aug 17;158(3-4):237-46. doi: 10.1016/j.vetmic.2012.01.021. Epub 2012 Jan 25.
Since 2006, highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) has become the major pathogen attributed to the prevalent porcine reproductive and respiratory syndrome (PRRS) in China. The present study aims to identify serum proteins modified in response to infection of HuN4, a HP-PRRSV strain isolated from a farm in 2006. 2-D DIGE analysis allowed for the detection of 19 differentially expressed protein spots, of which 18 were identified by MALDI-TOF/TOF MS. These 18 spots represented for a total of 9 proteins (6 up-regulated and 3 down-regulated), most of which belonged to the acute phase proteins in swine and showed a trend of regression in the late phase of the experiment. One of a series of AGP spots was identified for the first time to be decreased in acute phase of PRRSV infection in swine. But the whole level of the protein in the serum did not show significant changes by Western blot. The rising tendency of Hp was confirmed by Western blot and ELISA. These altered proteins were probably involved in the inflammatory process triggered by HuN4 and in alleviating the oxidative damage occurring in the process. In summary, these results may provide new insights into understanding the mechanisms of HP-PRRSV infection.
自 2006 年以来，高致病性猪繁殖与呼吸综合征病毒（HP-PRRSV）已成为导致中国流行的猪繁殖与呼吸综合征（PRRS）的主要病原体。本研究旨在鉴定感染 HuN4（一种 2006 年从农场分离出的 HP-PRRSV 株）后发生改变的血清蛋白。2-D DIGE 分析检测到 19 个差异表达的蛋白斑点，其中 18 个通过 MALDI-TOF/TOF MS 鉴定。这 18 个斑点共代表 9 种蛋白质（6 种上调和 3 种下调），其中大多数属于猪的急性期蛋白，在实验后期呈下降趋势。一系列 AGP 斑点中的一个首次被鉴定为在猪 PRRSV 感染的急性期减少。但通过 Western blot 发现血清中该蛋白的整体水平没有明显变化。Western blot 和 ELISA 证实了 Hp 的上升趋势。这些改变的蛋白质可能参与了 HuN4 引发的炎症过程，并缓解了该过程中发生的氧化损伤。综上所述，这些结果可能为理解 HP-PRRSV 感染的机制提供新的见解。
