Shiseido Research Center, 2-2-1 Hayabuchi, Yokohama 224-8558, Japan.
Acta Derm Venereol. 2013 Mar 27;93(2):138-43. doi: 10.2340/00015555-1423.
The stratum corneum dehydrates after exogenous hydration due to skincare or bathing. In this study, sheets of stratum corneum were isolated from reconstructed human epidermis and the barrier function and structure of these sheets were assessed during drying with the aim of improving our understanding of skincare. Water diffusion through the sheets of stratum corneum decreased with drying, accompanied by decreased thickness and increased visible light transmission through the sheets. Electron paramagnetic resonance revealed that the order parameter values of stratum corneum lipids increased with drying. X-ray diffraction analysis revealed increases in the diffraction intensity of lamellar structures, with an 11-12 nm periodicity and spacing of 0.42 nm for lattice structures with drying. These results suggest that the drying process improves the barrier function of the stratum corneum by organizing the intercellular lipids in a vertically compressed arrangement.
角质层在外用护肤品或沐浴后会因脱水而干燥。在这项研究中，从重建的人体表皮中分离出角质层薄片，并在干燥过程中评估这些薄片的屏障功能和结构，旨在增进我们对护肤品的理解。角质层薄片中的水分扩散随着干燥而减少，同时薄片的厚度减小，可见光透过率增加。电子顺磁共振显示，角质层脂质的有序参数值随着干燥而增加。X 射线衍射分析显示，随着干燥，层状结构的衍射强度增加，具有 11-12nm 周期性和 0.42nm 晶格结构间距。这些结果表明，干燥过程通过将细胞间脂质排列成垂直压缩的方式来改善角质层的屏障功能。
