在单分子水平上使用 SNAP/CLIP 标签介导的翻译进行 SUMOylation 的同时测量。

Simultaneous measurement of SUMOylation using SNAP/CLIP-tag-mediated translation at the single-molecule level.

机构信息

Single-molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Guangdong, China.

出版信息

Angew Chem Int Ed Engl. 2013 Jan 7;52(2):691-4. doi: 10.1002/anie.201206695. Epub 2012 Nov 21.

DOI:10.1002/anie.201206695

PMID:23169684

Abstract

Two opposing SUMO wrestlers: simultaneous measurement of multiple SUMOylations was achieved at the single-molecule level by integrating SNAP/CLIP-tag-mediated translation with single-molecule detection (SUMO=small ubiquitin-like modifier). This method gives exceptional sensitivity, and is capable of measuring the SUMOylation of different proteins under various physiological conditions.

摘要

两个对立的 SUMO 摔跤手：通过整合 SNAP/CLIP 标记介导的翻译与单分子检测，在单分子水平上实现了多种 SUMO 化的同时测量（SUMO=小泛素样修饰物）。这种方法具有出色的灵敏度，能够在各种生理条件下测量不同蛋白质的 SUMO 化。