CA Ingeniería de Biosistemas, División de Investigación y Posgrado, Facultad de Ingeniería, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Santiago de Querétaro, Qro., 76010, México.
Viruses. 2012 Sep;4(9):1792-801. doi: 10.3390/v4091792. Epub 2012 Sep 24.
The Geminiviruses genome is a small, single strand DNA that replicates in the plant cell nucleus. Analogous to animal DNA viruses, Geminiviruses depend on the host replication machinery to amplify their genomes and only supply the factors required to initiate their replication. Consequently, Geminiviruses remove the cell-cycle arrest and induce the host replication machinery using an endocycle process. They encode proteins, such as the conserved replication-associated proteins (Rep) that interact with retinoblastoma-like proteins in plants and alter the cell division cycle in yeasts. Therefore, the aim of this work is to analyze the impact of Pepper Golden Mosaic Virus (PepGMV) Rep protein in mammalian cells. Results indicate that the pTracer-SV40:Rep construction obtained in this work can be used to analyze the Rep protein effect in mammalian cells in order to compare the cell cycle regulation mechanisms in plants and animals.
双生病毒的基因组是一个小型的单链 DNA，在植物细胞核内复制。与动物 DNA 病毒类似，双生病毒依赖于宿主复制机制来扩增其基因组，并且只提供启动其复制所需的因素。因此，双生病毒通过内圈过程去除细胞周期停滞并诱导宿主复制机制。它们编码蛋白质，例如保守的复制相关蛋白 (Rep)，这些蛋白与植物中的视网膜母细胞瘤样蛋白相互作用，并改变酵母中的细胞分裂周期。因此，这项工作的目的是分析胡椒金黄镶嵌病毒 (PepGMV) Rep 蛋白在哺乳动物细胞中的作用。结果表明，本工作中获得的 pTracer-SV40:Rep 构建体可用于分析哺乳动物细胞中 Rep 蛋白的作用，以便比较植物和动物中细胞周期调控机制。
