Zou Jie, Guan Zheng, Zhang Wei-yi
Department of Obstetrics and Gynecology, the Chinese PLA General Hospital, Beijing.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2012 Aug;32(8):1112-6.
To compare the effects of sanjie zhentong capsule (SZC) and danazol on rats with endometriosis (EMT).
Totally 48 adult female Lewis rats were selected, 12 as the blank control group, and the rest 36 rats in the estrus cycle were used to establish the EMT model. After modeling they were randomly divided into 3 groups, i.e., the model control group, the SZC treatment group, and the danazol treatment group, 12 in each group. Four weeks later the focus was measured by a second laparotomy. The normal saline at 1 mL/day was administered to rats in the model control group, SZC at 86.4 mg/day to those in the SZC treatment group, and danazol at 7.2 mg/day to those in the danazol treatment group. All the treatment lasted for 4 weeks. At the end of the treatment, a third laparotomy was performed to measure the size of focus. The expression of proliferating cell nuclear antigen (PCNA) was detected using immunohistochemical assay. The cell apoptosis rate was detected using terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick-end labeling (TUNEL). The concentration of prostaglandin E2 (PGE2) in the peritoneal fluid and the serum were detected using ELISA.
There was statistical difference in the change of the focus volume between the SZC treatment group (-23.27 +/- 18.18) and the danazol treatment group (-12.28 +/- 10.04) and the model control group (13.97 +/- 7.54, P < 0.01). The expression of ectopic PCNA significantly decreased and the positive expression rate of TUNEL obviously increased in the two treatment groups when compared with the model control group (P < 0.05, P < 0.01). The expression of ectopic PCNA decreased and the positive expression rate of TUNEL increased more obviously in the SZC treatment group than in the danazol treatment group (P < 0.01). The concentration of PGE2 in the peritoneal fluid and the serum was significantly lower in the two treatment group when compared with the model control group (P < 0.01). The concentration of PGE2 in the peritoneal fluid and the serum was significantly lower in the SZC treatment group than in the danazol treatment group (P < 0.01).
SZC and danazol both could inhibit the focus growth in EMT rats. SZC showed better effects. It was an effective drug for treating EMT.
比较散结镇痛胶囊（SZC）与达那唑对子宫内膜异位症（EMT）大鼠的影响。
选取48只成年雌性Lewis大鼠，12只为空白对照组，其余36只处于发情周期的大鼠用于建立EMT模型。造模后将其随机分为3组，即模型对照组、SZC治疗组和达那唑治疗组，每组12只。4周后通过第二次剖腹术测量病灶大小。模型对照组大鼠每天给予1 mL生理盐水，SZC治疗组大鼠每天给予86.4 mg SZC，达那唑治疗组大鼠每天给予7.2 mg达那唑。所有治疗持续4周。治疗结束时，进行第三次剖腹术测量病灶大小。采用免疫组织化学法检测增殖细胞核抗原（PCNA）的表达。采用末端脱氧核苷酸转移酶介导的脱氧UTP缺口末端标记法（TUNEL）检测细胞凋亡率。采用酶联免疫吸附测定法（ELISA）检测腹腔液和血清中前列腺素E2（PGE2）的浓度。
SZC治疗组（-23.27±18.18）和达那唑治疗组（-12.28±10.04）病灶体积变化与模型对照组（13.97±7.54）相比，差异有统计学意义（P<0.01）。与模型对照组相比，两个治疗组异位PCNA表达显著降低，TUNEL阳性表达率明显升高（P<0.05，P<0.01）。SZC治疗组异位PCNA表达降低及TUNEL阳性表达率升高比达那唑治疗组更明显（P<0.01）。与模型对照组相比，两个治疗组腹腔液和血清中PGE2浓度显著降低（P<0.01）。SZC治疗组腹腔液和血清中PGE2浓度比达那唑治疗组显著降低（P<0.01）。
SZC和达那唑均可抑制EMT大鼠病灶生长。SZC效果更佳。它是治疗EMT的有效药物。
