Imani Rana, Hojjati Emami Shahriar, Fakhrzadeh Hossein, Baheiraei Nafiseh, Sharifi Ali M
Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
Biocell. 2012 Apr;36(1):37-45.
The ultimate goal of tissue engineering is to design and fabricate functional human tissues that are similar to natural cells and are capable of regeneration. Preparation of cell aggregates is one of the important steps in 3D tissue engineering technology, particularly in organ printing. Two simple methods, hanging drop (HD) and conical tube (CT) were utilized to prepare cell aggregates. The size and viability of the aggregates obtained at different initial cell densities and pre-culture duration were compared. The proliferative ability of the cell aggregates and their ability to spread in culture plates were also investigated. In both methods, the optimum average size of the aggregates was less than 500 microm. CT aggregates were smaller than HD aggregates. 5,000 cells per drop HD aggregates showed a marked ability to attach and spread on the culture surface. The proliferative ability reduced when the initial cell density was increased. Comparing these methods, we found that the HD method having better size controlling ability as well as enhanced ability to maintain higher rates of viability, spreading, and proliferation. In conclusion, smaller HD aggregates might be a suitable choice as building blocks for making bioink particles in bioprinting technique.
组织工程的最终目标是设计和制造与天然细胞相似且能够再生的功能性人体组织。制备细胞聚集体是三维组织工程技术中的重要步骤之一，尤其是在器官打印方面。采用两种简单方法，即悬滴法（HD）和锥形管法（CT）来制备细胞聚集体。比较了在不同初始细胞密度和预培养时间下获得的聚集体的大小和活力。还研究了细胞聚集体的增殖能力及其在培养板中铺展的能力。在两种方法中，聚集体的最佳平均尺寸均小于500微米。CT聚集体比HD聚集体小。每滴含5000个细胞的HD聚集体在培养表面显示出明显的附着和铺展能力。当初始细胞密度增加时，增殖能力降低。比较这些方法，我们发现HD方法具有更好的尺寸控制能力以及更高的维持活力、铺展和增殖率的能力。总之，较小的HD聚集体可能是生物打印技术中制造生物墨水颗粒的合适构建块选择。
