Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
Peptides. 2013 Jan;39:131-7. doi: 10.1016/j.peptides.2012.10.016. Epub 2012 Nov 19.
Putative antifungal peptide encoding genes containing Penicillium chrysogenum antifungal protein (PAF) characteristic amino acid motifs were identified in 15 Fusarium isolates, representing 10 species. Based on the predicted sequences of mature peptides, discrepancy in one, two or three amino acids was observed between them. Phylogenetic investigations revealed that they show high amino acid sequence similarity to PAF and they belong to the group of fungal derived antifungal peptides with PAF-cluster. Ten from the 15 partially purified <10 kDa peptide fraction of Fusarium ferment broths showed antifungal activity. The presence of approximately 6.3 kDa molecular weight peptides was detected in all of the antifungally active ferment broths, and this peptide was isolated and purified from Fusarium polyphilaidicum. The minimal inhibitiory concentrations of F. polyphilaidicum antifungal protein (FPAP) were determined against different filamentous fungi, yeasts and bacteria. Filamentous fungal species were the most susceptible to FPAF, but some yeasts were also slightly sensitive.
在 15 个代表 10 个种的镰刀菌分离物中鉴定到含有青霉素蛋白 (PAF) 特征性氨基酸模体的假定抗真菌肽编码基因。基于成熟肽的预测序列，它们之间观察到一个、两个或三个氨基酸的差异。系统发育研究表明，它们与 PAF 具有很高的氨基酸序列相似性，属于具有 PAF 簇的真菌来源的抗真菌肽组。在 15 个部分纯化的<10 kDa 镰刀菌发酵液肽级分中，有 10 个显示出抗真菌活性。在所有具有抗真菌活性的发酵液中都检测到约 6.3 kDa 分子量的肽，该肽从多形被毛孢中分离和纯化。测定了多形被毛孢抗真菌蛋白 (FPAP) 对不同丝状真菌、酵母和细菌的最小抑菌浓度。丝状真菌物种对 FPAF 最敏感，但一些酵母也有轻微的敏感性。
