Zhang Zhaoxia, Zhao Xinmei, Lv Chaolan, Li Chen, Zhi Fachao
Department of Gastroenterology, Southern Medical University, Guangzhou, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2012 Nov;32(11):1548-52.
To explore the role of annexin A2 (ANXA2) expression in the intestinal mucosa in the pathogenesis of inflammatory bowel disease (IBD).
Intestinal or colonic mucosal biopsy samples were obtained from 54 patients with ulcerative colitis (UC), 37 with Crohn's disease (CD), and 15 healthy control subjects. Immunohistochemistry was employed to examine the expression of ANXA2 in the intestinal mucosa, and mRNA expression of ANXA2 was detected using real-time PCR.
Immunohistochemistry showed a ANXA2 positivity rate of 83.3% (45/54) in patients with UC, 27.0% (10/37) in patients with CD, and 53.3% (8/15) in the control subjects. ANXA2 expression in the intestinal or colonic mucosa was significantly up-regulated in patients with UC compared with the patients with CD and healthy control subjects, but was significantly lower in patients with CD than in the healthy controls (P<0.05). The expression levels of ANXA2 were strongly associated with the severity of clinical manifestations and the histopathological grades of UC (P<0.05). Compared with the healthy controls and patients with CD, patients with UC showed a significantly increased ANXA2 mRNA expression level in the inflamed mucosa of UC (P<0.05).
ANXA2 can serve as a marker for differential diagnosis of IBD, and its up-regulated expression is closely related to the pathogenesis of UC.
探讨膜联蛋白A2（ANXA2）在肠黏膜中的表达在炎症性肠病（IBD）发病机制中的作用。
从54例溃疡性结肠炎（UC）患者、37例克罗恩病（CD）患者和15名健康对照者中获取肠道或结肠黏膜活检样本。采用免疫组织化学法检测肠黏膜中ANXA2的表达，并使用实时荧光定量PCR检测ANXA2的mRNA表达。
免疫组织化学显示，UC患者中ANXA2阳性率为83.3%（45/54），CD患者中为27.0%（10/37），对照者中为53.3%（8/15）。与CD患者和健康对照者相比，UC患者肠或结肠黏膜中ANXA2表达显著上调，但CD患者中ANXA2表达显著低于健康对照者（P<0.05）。ANXA2表达水平与UC临床表现严重程度及组织病理学分级密切相关（P<0.05）。与健康对照者和CD患者相比，UC患者炎症黏膜中ANXA2 mRNA表达水平显著升高（P<0.05）。
ANXA2可作为IBD鉴别诊断的标志物，其表达上调与UC发病机制密切相关。
