Nałecz K A, Müller M, Zambrowicz E B, Wojtczak L, Azzi A
Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland.
Biochim Biophys Acta. 1990 Apr 5;1016(2):272-9. doi: 10.1016/0005-2728(90)90069-g.
The role and properties of -SH groups of purified pyruvate (monocarboxylate) carrier were investigated. After isolation, this protein has all -SH groups in the oxidized state. Upon reduction, the carrier can be labelled with eosin-5-maleimide. The shift in apparent Mr after the labelling points to the presence of at least two cysteine residues. Pyruvate uptake in the reconstituted system is inhibited by both permeable (eosin-5-maleimide at 1 mM concentration) and impermeable (mersalyl, p-chloromercuribenzoate) -SH group reagents. Phenylarsine oxide inhibits pyruvate transport only slightly (20%), but the inhibition is enhanced after preincubation with the substrate.
研究了纯化的丙酮酸（单羧酸盐）载体中-SH基团的作用和性质。分离后，该蛋白的所有-SH基团均处于氧化状态。还原后，载体可用曙红-5-马来酰亚胺标记。标记后表观分子量的变化表明至少存在两个半胱氨酸残基。在重构系统中，丙酮酸摄取受到可渗透的（1 mM浓度的曙红-5-马来酰亚胺）和不可渗透的（汞撒利、对氯汞苯甲酸）-SH基团试剂的抑制。苯胂氧化物仅轻微抑制丙酮酸转运（20%），但在与底物预孵育后抑制作用增强。
