Nałecz K A, Zambrowicz B E
Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warszawa, Poland.
Acta Biochim Pol. 1990;37(1):109-12.
A method has been developed for functional reconstitution of the isolated pyruvate carrier. Optimal conditions were reached after removal of deoxycholate with 27 mg Triton X-100 per gram of moist Amberlite XAD-2 beads and 7 micrograms protein/mg of phospholipid. Uptake of pyruvate into proteoliposomes and sensitivity of the carrier towards inhibitors resemble those in intact mitochondria.
已开发出一种用于分离的丙酮酸载体功能重构的方法。在用每克湿的Amberlite XAD - 2珠粒27毫克Triton X - 100和每毫克磷脂7微克蛋白质去除脱氧胆酸盐后达到最佳条件。丙酮酸摄入蛋白脂质体以及载体对抑制剂的敏感性类似于完整线粒体中的情况。
