Monocarboxylate carrier from bovine heart mitochondria: reconstitution in a functionally active state.

A method has been developed for functional reconstitution of the isolated pyruvate carrier. Optimal conditions were reached after removal of deoxycholate with 27 mg Triton X-100 per gram of moist Amberlite XAD-2 beads and 7 micrograms protein/mg of phospholipid. Uptake of pyruvate into proteoliposomes and sensitivity of the carrier towards inhibitors resemble those in intact mitochondria.