猪黄体线粒体内膜的组成。

Composition of the inner mitochondrial membrane of porcine corpus luteum.

作者信息

Holland J W, Stevenson P M

机构信息

Department of Biochemistry, University of Western Australia, Nedlands, Perth.

出版信息

Biochim Biophys Acta. 1990 Mar;1022(3):401-7. doi: 10.1016/0005-2736(90)90291-u.

DOI:10.1016/0005-2736(90)90291-u

PMID:2317488

Abstract

An inner mitochondrial membrane fraction was prepared from porcine corpus luteum. The concentrations of the respiratory cytochromes, cytochrome P-450scc, cholesterol, ubiquinone, cardiolipin and the total phospholipids were measured. The fatty acid compositions of cardiolipin and the total phospholipid fraction were determined. Comparative data from porcine heart and liver were obtained using the same methods. Differences in both the concentration and the fatty acid composition of the phospholipids were observed between the tissues. It appeared that the phospholipid bilayer was expanded relative to haem a in luteal mitochondria. It is proposed that in the ovary this expansion may be necessary to accommodate cytochrome P-450scc and its substrate, cholesterol.

摘要

从猪黄体中制备线粒体内膜组分。测定呼吸细胞色素、细胞色素P-450scc、胆固醇、泛醌、心磷脂和总磷脂的浓度。测定心磷脂和总磷脂组分的脂肪酸组成。使用相同方法获得猪心脏和肝脏的比较数据。观察到不同组织间磷脂浓度和脂肪酸组成存在差异。黄体线粒体中磷脂双层相对于血红素a似乎有所扩展。有人提出，在卵巢中这种扩展可能是为了容纳细胞色素P-450scc及其底物胆固醇。