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植物细胞中心磷脂的精确定位。

A precise localization of cardiolipin in plant cells.

作者信息

Bligny R, Douce R

出版信息

Biochim Biophys Acta. 1980 Feb 22;617(2):254-63. doi: 10.1016/0005-2760(80)90168-x.

DOI:10.1016/0005-2760(80)90168-x
PMID:6243994
Abstract

Cardiolipin and cytochrome aa3 contents of isolated plant cells (sycamore cells) and their purified mitochondria were measured. Since the cardiolipin/cytochrome aa3 ratio was the same in the intact cells and in the isolated mitochondria it was strongly suggested that cardiolipin is present only in the mitochondria. Furthermore, outer and inner mitochondria membranes of purified sycamore cells and mung bean hypocotyl mitochondria were separated and it was shown that cardiolipin is localized in the inner mitochondrial membrane.

摘要

对分离出的植物细胞(悬铃木细胞)及其纯化的线粒体中的心磷脂和细胞色素aa3含量进行了测定。由于完整细胞和分离出线粒体中的心磷脂/细胞色素aa3比值相同,因此强烈表明心磷脂仅存在于线粒体中。此外,对纯化的悬铃木细胞和绿豆下胚轴线粒体的线粒体外膜和内膜进行了分离,结果表明心磷脂定位于线粒体内膜。

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