在血清替代品Ultroser G存在的情况下，人皮肤成纤维细胞在微载体上的培养

The culture of human skin fibroblasts on microcarriers in the presence of serum substitute: Ultroser G.

作者信息

Miech G, Myara I, Mangeot M, Gautier M, Lemonnier A

机构信息

Laboratoire de Biochimie Appliquée, Faculté de Pharmacie, Chatenay-Malabry, France.

出版信息

Biol Cell. 1990;68(1):85-7. doi: 10.1111/j.1768-322x.1990.tb00897.x.

DOI:10.1111/j.1768-322x.1990.tb00897.x

PMID:2317596

Abstract

The effects of the substitution of serum by Ultroser G on human skin fibroblasts cultured on microcarriers were analysed. Cultures could not be established on microcarriers in the presence of Ultroser G. However, microcarrier cultures started in the presence of 10% foetal calf serum, and transferred to 2% Ultroser G after 7 days resulted in high cell densities.

摘要

分析了用Ultroser G替代血清对在微载体上培养的人皮肤成纤维细胞的影响。在存在Ultroser G的情况下无法在微载体上建立培养物。然而，在10%胎牛血清存在下开始的微载体培养物，并在7天后转移到2%的Ultroser G中，可得到高细胞密度。