血清替代物Ultroser G在体外哺乳动物细胞毒理学评估中的影响。
The influence of serum substitute Ultroser G in toxicological evaluations in mammalian cells in vitro.
作者信息
Rodrigues M A, Mattei R
机构信息
Instituto Biológico, Sào Paulo, Brazil.
出版信息
Ecotoxicol Environ Saf. 1987 Dec;14(3):269-74. doi: 10.1016/0147-6513(87)90070-4.
The influence of serum substitute Ultroser G on the toxicity of the antiparasitic ivermectin was evaluated in CHO and IB-RS-2 pig kidney cells. The growth of both cell lines was slightly slower in medium with Ultroser G than with serum. However, the growth of both CHO and IB-RS-2 cells exposed to 5 and 10 micrograms/ml ivermectin decreased to a higher extent when they were maintained in medium with Ultroser G than with serum. The treatment with 20 micrograms/ml caused the death of the cells cultured with the serum substitute within 6 hr; the growth of CHO cells ceased after 24 hr in medium with serum, while IB-RS-2 cells were still slowly growing after 72 hr. The morphological changes caused by the drug were observed under light microscope.
在CHO和IB-RS-2猪肾细胞中评估了血清替代品Ultroser G对抗寄生虫药物伊维菌素毒性的影响。在含有Ultroser G的培养基中，这两种细胞系的生长均比在含有血清的培养基中略慢。然而，当CHO和IB-RS-2细胞暴露于5和10微克/毫升伊维菌素时，与在含有血清的培养基中相比，它们在含有Ultroser G的培养基中生长下降的程度更高。用20微克/毫升处理会导致在含有血清替代品的培养基中培养的细胞在6小时内死亡；在含有血清的培养基中，CHO细胞的生长在24小时后停止，而IB-RS-2细胞在72小时后仍在缓慢生长。在光学显微镜下观察了药物引起的形态变化。
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