Department of Analytical Chemistry and Pharmaceutical Technology, Center for Pharmaceutical Research, Vrije Universiteit Brussel-VUB, Laarbeeklaan 103, B-1090 Brussels, Belgium.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Dec 1;910:103-13. doi: 10.1016/j.jchromb.2012.10.001. Epub 2012 Oct 26.
The Mallotus genus comprises numerous species used as traditional medicines in oriental countries and provides scientists a broad basis in the search for pharmacologically active constituents. In this paper, the cytotoxicity of 39 Mallotus extracts, different in species, part of the plant used, origin, and harvest season, is evaluated combining cytotoxicity assays with fingerprint technology and data handling tools. At first, the antiproliferative activity of the plant extracts is analyzed both on a non-cancerous cell line (WI-38--human lung fibroblast) and on a cancerous cell line (HeLa human cervix carcinoma). The results are linked to a data set of high-performance liquid chromatographic fingerprint profiles of the samples using multivariate calibration techniques. The regression coefficients of the multivariate model are then evaluated to indicate those peaks potentially responsible for the cytotoxic activity of the Mallotus extracts. In a final step, the cytotoxic extracts are analyzed by HPLC-MS and the indicated peaks identified.
麻疯树属包含许多种被用作东方国家传统药物的物种，为科学家寻找具有药理活性的成分提供了广泛的基础。在本文中，通过结合细胞毒性测定、指纹图谱技术和数据处理工具，评估了 39 种不同物种、植物部位、来源和收获季节的麻疯树属提取物的细胞毒性。首先，在非癌细胞系（WI-38-人肺成纤维细胞）和癌细胞系（HeLa 人宫颈癌细胞）上分析植物提取物的抗增殖活性。将结果与样品的高效液相色谱指纹图谱数据集相关联，使用多元校准技术。然后评估多元模型的回归系数，以指示那些可能对麻疯树属提取物的细胞毒性活性负责的峰。在最后一步，通过 HPLC-MS 分析细胞毒性提取物，并鉴定出所示的峰。
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