Key Lab of Marine Bioactive Substances, First Institute of Oceanography, SOA, Xianxialing Road 6, Qingdao, 266061, China.
Curr Microbiol. 2013 Mar;66(3):259-65. doi: 10.1007/s00284-012-0266-5. Epub 2012 Nov 24.
An OmpR gene, named OmpR503, was cloned from the Antarctic psychrotrophic bacterium Psychrobacter sp. G according to its genomic draft. The deduced amino acid sequences of OmpR503 were highly conserved with other known protein members of OmpR family. qRT-PCR analysis showed that the expression of OmpR503 gene was significantly enhanced by high salinity (90, 120). The expression of OmpR503 gene was also significantly increased at low temperature (0, 10 °C), whereas depressed at high temperature (30 °C). When the strain was subjected to combined stress (0 °C with a salinity of 90), the expression of OmpR503 gene was increased significantly, which was up to 3.0-fold. In Antarctica, freezing tolerance of psychrotrophic bacteria is often accompanied by tolerance to osmotic stress caused by a lack of free water, thus the cold inducibility of OmpR503 gene might help the strain adapt to the harsh environment more efficiently.
根据南极嗜冷菌 Psychrobacter sp. G 的基因组草图，从该菌中克隆出一个 OmpR 基因，命名为 OmpR503。OmpR503 的推导氨基酸序列与其他已知的 OmpR 家族蛋白成员高度保守。qRT-PCR 分析表明，OmpR503 基因的表达受高盐度（90、120）显著增强。OmpR503 基因的表达在低温（0、10°C）下也显著增加，而在高温（30°C）下则受到抑制。当菌株受到复合胁迫（0°C 加 90 的盐度）时，OmpR503 基因的表达显著增加，达到 3.0 倍。在南极洲，嗜冷菌的抗冻性通常伴随着对由于缺乏游离水而导致的渗透压胁迫的耐受性，因此 OmpR503 基因的冷诱导性可能有助于该菌株更有效地适应恶劣环境。
